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Standard operating procedure
24 Hour urine collections No: 010D
24 Hour urine collections provide vital information in the further
investigation of hypertensive subjects. They provide a quantitative
measurement of the subject's sodium and potassium excretion,
microalbuminuria and estimation of creatinine clearance.
Research nurses trained in the method are responsible for teaching
subjects how to collect their urine efficiently.
- 24 hour
plastic urine container
- 1 litre
- Low temperature
marker pens & label
fluids should be considered as potentially infectious material
and should be handled accordingly. All 24 hour urine collections
should be collected using the following guidelines:-
Preparation of subject
to the subject the importance of the 24 hour urine collection.
Ensure that they understand that 1 missed sample will impair/invalidate
the results obtained from that collection.
what the equipment is for and the time when the 24 hour urine
collection will start and finish.
Preparation of materials
that the plastic urine container is empty and that the bottle
is plain and Does not contain any acid.
that the bottle is labelled correctly with all the subject's
that the subject has an instruction sheet, with the space
provided on it, to write down the exact start and finish time
of the collection.
24 hour collection
- The 24
hour urine collection usually starts in the morning. After
the subject has awoken, they first urinate as usual down the
toilet and note the time on their instruction sheet. The collection
time STARTS from this time, that is the time they have first
urinated into the toilet and their bladder is then empty.
Instruct the subject that every time they now need to go to
the toilet they must take their plastic jug to urinate into.
When finished, instruct them to pour the urine content of
the jug efficiently (without any spillage) into the 24 hour
urine collection container.
the subject to store the container in a cool, dry environment
until it is required for another specimen.
the subject to rinse the plastic jug out and hand dry it until
that the subject is aware that the urine collection finishes
at the same time point, 24 hours after it commenced. 5 minutes
prior to the end time, the subject should be instructed to
empty their bladder even if they do not have the urge to go.
This urine (having been placed in the container) then completes
- The subject
should then be instructed to return the urine collection as
soon after completing it as possible to their research centre.
If possible the urine should be stored in a cool dry place
(refrigerator if available) until analysed.
the total volume along with the subject's details in the case
4 x 50ml aliquots of the urine collection for storage and
freeze it at -20°C. Ensure the urine containers are suitably
labelled by using the low temperature marker pens. They should
clearly indicate subject and study identification codes.
the actual times and any comments or information in the subjects
case report file for future reference.
- A handy
reminder for men, is to instruct them to put a safety pin
in their trouser fly as a useful reminder.
working and the prevention of infection in clinical laboratories
- model rules for staff and visitors (HSE).