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Recruitment for the sibling pair study was initiated in late 1996. The aim was to recruit 1500 white
European families based upon affected sibling pairs in order to map genes causing high blood pressure. (Demographics of the collected rescource)
At the out-set
six recruitment centres were established and standard operating
procedures in phenotyping, genetic counselling and genotyping
were set up to ensure consistency between the sites.
participated as volunteers and were recruited via hypertension
registers from the MRC General Practice Framework and Scottish
European, British origin (up to level of grand-parental ancestry).
Men and women aged between 18-85 years with known hypertension.
age of diagnosis of hypertension for the proband had to be less
than 50 years and the sibling less than or equal to 60 years.
Both affected siblings in each family had to have blood pressure
recordings of 150/100mmHg using 1 reading, or 145/95mmHg, mean
of 3 readings whilst seated.
Both affected siblings had to meet
blood pressure and age of onset criteria to be included in the
Affected individuals who consumed more
than 21 units of alcohol per week.
Obese individuals defined as a BMI of 30 Kg/m2 or greater.
Diabetes (these may represent a
Individuals with intrinsic renal disease.
Individuals with a history of secondary hypertension, or co-existing illness,
which could confound accurate phenotyping.
Socio-economic, personal medical history, genealogy, family
history of cardiovascular disease and medication were all recorded.
blood pressure recordings using the Omron HEM-705CP, portable,
semi-automated oscillometric device (Omron Healthcare).
ambulatory blood pressure recordings (Spacelabs, Model 90207).
Anthropometric measurements (waist-hip ratio, skin fold
thickness and body mass index).
A 12-lead electrocardiogram
(ECG) was performed.
Each participant was offered
Samples, biochemistry and urinary analysis:
Venous blood was
collected for biochemical analysis, DNA extraction and peripheral
blood lymphocyte preparation.
Blood glucose concentration, total
cholesterol, triglycerides, g-GT, urate, and electrolytes were
measured at a single central chemical pathology lab in Glasgow
(Members of National Quality Assurance Scheme, CPA accredited).
A 24-hour urine sample was also collected and from this 24-hour
creatinine clearance, urinary electrolytes and albumin/creatinine
ratio were measured.
Data protection and databases:
A unique family identification number
was given to each individual and subsequently blood, urine and DNA samples were stored using
Computerised records of all samples conformed
to the Data Protection Act.
and genetic data is stored in a password protected MySQL relational database. To ensure accuracy and data quality control
we employed a double data entry system whereby 2 independently
trained staff entered all the phenotypic information, a third
person reconciled any inconsistencies. Entry of biochemical
results and Minnesota coded ECGs was by direct electronic transfer.
The BRIGHT study sib pair recruitment was extremely successful and comprises 1708 families based upon sibling pairs.
Demographics of the sibling pair rescource